PrimerSuite

The precision lab companion. Validate binding, simulate thermodynamics, and automated benchtop calculations in one seamless suite.

Target DNA Sequence (5' -> 3')

Forward Primer (5' -> 3')

Reverse Primer (5' -> 3')

Analysis Results

Forward Primer

Tm: -

GC%: -

Length: -

Reverse Primer

Tm: -

GC%: -

Length: -

PCR Product

Amplicon size estimates and binding region.

Product Size -

Annealing Ta -

Visualization will appear here

Master Mix Calculator

Samples (N)

Vol per Rxn (µL)

Add 10% Dead Volume

Component	1 Rxn (µL)	Total (µL)
Water
Buffer (10X)
dNTPs (10mM)
Fwd Primer
Rev Primer
Taq Pol
DNA Template		N/A

Thermocycler Profile

Cycling Program AUTO-GEN

1. Init Denat 95°C for 2:00

2. Denature 95°C for 0:30

3. Anneal --°C for 0:30

4. Extend 72°C for --:--

Repeated 30-35x

5. Final Ext 72°C for 5:00

6. Hold 4°C

Based on --- bp amplicon & ---°C Ta

How to use PrimerSuite

Validate Design

Paste your Target DNA and Primer sequences. We check for binding specificity (`Tm`), self-dimers, and hairpins (`ΔG`). Use the Visualizer to see exactly how primers anneal.

Plan the Bench

Switch to the Benchtop tab. Enter your sample count ($N$) to get an instant Master Mix recipe. We also generate a starting Thermocycler Profile customized to your product size.

Batch Process

Have 96 pairs? Go to Batch Mode. Upload a simple CSV (`Target, Fwd, Rev`) and validate the entire plate in seconds. Results export immediately to Excel/CSV.

Primer Validator